Tonight (May 25th), Zhongguancun Forum 2023 opened in Beijing. At the opening ceremony, ten major scientific and technological achievements in the construction of Beijing International Science and Technology Innovation Center were released.

　　Major scientific and technological achievement 1: Evaluation report on the construction of Beijing International Science and Technology Innovation Center

　　Beijing International Science and Technology Innovation Center is accelerating its construction. Entrusted by the Ministry of Science and Technology, China Academy of Engineering fully evaluated the construction of Beijing International Science and Technology Innovation Center, and formed an evaluation report. According to the assessment, Beijing’s original innovation and the supply capacity of science and technology sources have been improved, and a demonstration exploration has been made in establishing a new national system, and a science and technology innovation center with global influence has been initially established.

　　Major scientific and technological achievements II: Important progress and achievements of Zhongguancun’s pilot reform.

　　In November, 2021, the 22nd meeting of the Central Committee for Comprehensively Deepening Reform deliberated and adopted the "Several Measures on Supporting Zhongguancun National Independent Innovation Demonstration Zone to Carry out High-level Scientific and Technological Self-reliance and Self-improvement", and took the lead in exploring a series of major reform measures in the core area of Zhongguancun. At present, a total of 58 supporting policies have been issued, and important progress has been made and obvious results have been achieved.

　　The motivation of R&D investment of enterprises has been greatly improved, and more than 10,000 enterprises have enjoyed the policies of basic research tax pilot and the proportion of R&D expenses of small and medium-sized scientific and technological enterprises has been increased to 100%, with an additional deduction of more than 30 billion yuan.

　　Industry-University-Research’s deep integration has been continuously strengthened, and the "use first and pay later" pilot of scientific and technological achievements has attracted nearly 1,000 patent services from universities and research institutes to innovate. Support the formation of a group of innovative consortia led by leading scientific and technological enterprises.

　　The training and introduction of talents have been further strengthened, and the joint training of graduate students by new R&D institutions and universities has been normalized.

　　Zhongguancun’s pilot reform measures will be promoted to the whole demonstration area.

　　Major scientific and technological achievement 3: a new generation of 256-core blockchain dedicated acceleration chip

　　Beijing Microchip Blockchain and Edge Computing Research Institute strengthened the original innovation and successfully developed a new generation of 256-core high-performance blockchain acceleration chips. This chip integrates strong computing power and high security, dispatches 256 cores for multi-thread concurrent operation, can handle 1 million blockchain intelligent contract transactions per second, and provides efficient privacy computing capability to realize "data is available and invisible", which can support the construction of a new digital infrastructure with high performance, high credibility and high security.

　　The fourth major scientific and technological achievement: ultra-fast instantaneous energy band regulation of semiconductor black phosphorus

　　The interaction between light and matter is an important means to explore the micro-physical mechanism of low-dimensional quantum materials such as graphene, topology and superconductivity, among which ultra-short and ultra-intense pulsed laser can also be used as an effective means to regulate the electronic structure and state of matter. Using femtosecond pulsed laser, Tsinghua University scientific research team filmed the "movie" of electronic structure evolution with time on the ultra-fast time scale of one trillionth of a second, and realized the measurement and instantaneous regulation of non-equilibrium electronic structure. Using this technology, the research team observed the instantaneous energy band regulation in the semiconductor material black phosphorus for the first time, that is, the electronic structure of black phosphorus evolved from the parabolic shape of equilibrium to the shape of "Mexican hat", and successfully rewritten the "gene" of black phosphorus by femtosecond laser. This important scientific discovery not only expands the frontier of non-equilibrium physics knowledge, but also lays a solid foundation for the future ultra-fast time scale regulation of material properties, which indicates that China has made important progress in the international frontier research field of non-equilibrium physics in condensed matter physics and material science.

　　Major scientific and technological achievement 5: reprogramming system of human cytochemistry

　　Pluripotent stem cells have unlimited self-renewal and differentiation into all functional cell types of organisms. These magical characteristics make them widely used in cell therapy, drug screening and other fields, and they are the most critical "seed cells" in the field of regenerative medicine. For the first time in the world, Peking University’s scientific research team achieved a major breakthrough in using small chemical molecules to regulate the fate of cells. Through the establishment of human cytochemical reprogramming method, the transformation of different somatic cell types was realized, human skin cells were transformed into pluripotent stem cells, and islet cells were successfully prepared. Compared with the traditional technology, the chemical reprogramming system is safer and simpler, easy to standardize and control, and solves the underlying technical problems in the development of stem cells and regenerative medicine.

　　Major scientific and technological achievement 6: a new generation of quantum computing cloud platform

　　Quantum computing cloud platform is the combination of quantum computer and cloud computing technology, and it is the display of comprehensive performance of quantum computing. A large-scale and stable quantum computing cloud platform was born in Beijing Institute of Quantum Information Science and named "Quantum Future -QUAFU". Superconducting quantum chips with 136, 18 and 10 qubits have been launched on the platform, which is the largest cloud platform with the highest number of bits per chip in China, and has achieved completely independent research and development and localization. The platform is compatible with the international standard of open quantum assembly language, and provides users with a quantum computing platform that is convenient for submitting computing tasks and carrying out important scientific research. The control and reading accuracy of the platform exceeds 98%, reaching the international advanced level.

　　Major scientific and technological achievement VII: escape mechanism of humoral immunity and characteristics of mutation evolution in Covid-19.

　　The research team of Changping Laboratory independently developed Qualcomm-based deep mutation scanning technology, which cracked the escape mechanism of various amino acid mutations of Omicron strain in COVID-19 to neutralizing antibodies, first revealed the phenomenon and principle of Covid-19’s convergent evolution, and constructed the evolution prediction model of Covid-19 receptor binding domain (RBD) for the first time in the world, and accurately predicted the future evolution trend of related strains, providing key theoretical and technical support for the development of antibody drugs and broad-spectrum vaccines.

　　Major scientific and technological achievement VIII: technological revolution and strategic breakthrough of continental shale oil

　　China Petroleum and Natural Gas Group Co., Ltd. innovated the geological theory of in-source enrichment of continental shale oil, established five key technical systems, such as experimental characterization of continental shale oil occurrence, evaluation of "dessert area/section", rotary steering optimal drilling, horizontal well volume fracturing development, and advanced reserve of underground shale heating conversion, and formed a number of independent intellectual property rights, including 11 national and industrial standards and 168 invention patents, and built three countries, including Gulong in Songliao Basin, Longdong in Ordos Basin and Jimsar in Junggar Basin.

　　Major scientific and technological achievements IX: China discipline and frontier field 2035 development strategy series

　　"China discipline and frontier field 2035 development strategy series" was officially released. The series was jointly organized by China Academy of Sciences and the National Natural Science Foundation of China, and invited more than 3,000 academicians and experts in related fields to study together. It took three years to form a series of 38 books, covering the development of China disciplines and frontier fields such as artificial intelligence, synthetic science and advanced materials, which will become an important reference for the development of science and technology.

　　Major scientific and technological achievement X: International scientific and technological organizations landed in Beijing

　　At present, international scientific and technological organizations such as International Hydrogen Fuel Cell Association, World Robotics Cooperation Organization, International Intelligent Manufacturing Alliance, International Intermediate Science Organization and Asian Simulation Alliance have successively landed in Beijing. Beijing will build an international science and technology organization headquarters cluster in Chaoyang District, attracting more international organizations to land and empowering the international science and technology innovation center to further integrate into the global innovation network.

　　(Headquarters reporter Wang Shengdong Yin Yinan)

　　CCTV News:The draft budget for 2018 announced by the Japan Coast Guard has reached a record high. In order to strengthen the so-called maritime security of the Diaoyu Islands, Japan has made a lot of tricks, not only allocating 39.4 billion yen to build new patrol boats and purchase aircraft, but also planning to introduce satellite surveillance systems.

　　According to Japanese media reports, the Japan Coast Guard announced the draft budget for fiscal year 2018 on the 29th, with a total amount of 230.3 billion yen (about 14 billion yuan), an increase of 9% compared with the original budget for 2017 and a record high. In addition, the staffing also plans to increase by 493 people next year. Japan’s Kyodo News Agency said that the Coast Guard plans to apply for a record 39.4 billion yen (about 2.4 billion yuan) to build a so-called "strategic maritime security system" to strengthen security near the Diaoyu Islands, including funding for a new 6,500-ton large patrol boat capable of carrying helicopters, a 1,500-ton medium patrol boat and nine small patrol boats, and plans to introduce a new jet plane with a long cruising range. Japanese TBS TV said that the new patrol boat is scheduled to officially sail in 2021.

The so-called "Diaoyu Island Exclusive Force" at Ishigaki Port in Okinawa was first exposed.

Chinese and Japanese law enforcement vessels have close contact in the waters around Diaoyu Island.

　　Around the Diaoyu Islands issue, the Japan Coast Guard launched the so-called "Diaoyu Islands Special Team System" consisting of 12 patrol boats in April 2016, equipped with 10 1,500-ton patrol boats and 2 large patrol boats capable of carrying helicopters. In March 2017, the so-called "Diaoyu Island Exclusive Force" in Ishigaki Port, Okinawa was first exposed. The Japan Coast Guard also released a video of close contact between Chinese and Japanese law enforcement vessels in the waters around the Diaoyu Islands.

　　Although the Japanese Coast Guard has concentrated the most elite forces in the direction of the Diaoyu Islands, the Japanese government is still in crisis in the face of the increasingly frequent cruise activities of China official ships in the Diaoyu Islands and its surrounding waters. According to the Yomiuri Shimbun, in 2012, there were only 68 official ships from China cruising in the territorial waters of Diaoyu Island, and by 2016, the number has increased to 121. China has comprehensively improved its maritime cruise capability in terms of quality and quantity.

　　Japanese media: The satellite system will help to quickly grasp the trend of China official ships.

　Japan coast guard patrol boat

　　At present, Japanese military strategic assets are accumulating in the Diaoyu Islands and the East China Sea, and the Coast Guard has become the "Depth Charge". By the end of 2016, Japan Coast Guard had nearly 370 patrol boats of various types, including 14 large patrol boats capable of carrying helicopters and 74 fixed-wing aircraft and helicopters.

　　According to Japan’s "Yomiuri Shimbun" reported on the 29th, Japan Coast Guard decided to introduce satellite surveillance system from this year. The monitoring object of the system ranges from the periphery of the Japanese archipelago to the coastal areas of China, South Korea and Russia, with an estimated area of 2.2 million square kilometers. The surveillance frequency of this area is at least twice a day, and the photos and videos taken by surveillance satellites will be transmitted to the headquarters of the national district of Japan for vigilance and search.

　　According to the report, so far, the Marine Insurance Agency has been responding to the patrol and search tasks by increasing patrol boats and planes. If it has its own satellite surveillance system, it can focus on shooting images of key surveillance areas and search for more accurate information. The introduction of this system will help to quickly grasp the movements of China official ships cruising in the territorial waters of Diaoyu Island.

　　Japanese media: The Japanese Police Agency will purchase more large helicopters for "seizing the island"

　　According to the Sankei Shimbun, the draft budget for fiscal year 2018 announced by the Japanese Police Agency on the 29th also contains contents related to the Diaoyu Islands. The cost of adding two large helicopters to the police in Fukuoka and Okinawa counties was added to the draft. This large helicopter can carry 25 people, which is the largest in police equipment. According to the report, the large helicopter can be used to deal with emergencies such as "foreign armed groups or groups claiming territorial sovereignty landing on outlying islands".

　　General Office of Hunan Provincial People’s Government

　　Notice on Issuing the Emergency Plan for Water Search and Rescue in Hunan Province

　　Xiang Zheng Ban Fa [2015] No.99

Municipal People’s governments, provincial government departments and commissions, and directly affiliated institutions:

　　"Emergency Plan for Water Search and Rescue in Hunan Province" has been agreed by the provincial people’s government and is hereby issued to you, please earnestly organize its implementation.

　　General Office of Hunan Provincial People’s Government

　　November 19, 2015

　　Emergency plan for water search and rescue in Hunan Province

　　Catalogue

　　1. General principles

　　1.1 Purpose of compilation

　　1.2 Compilation basis

　　1.3 Scope of application

　　1.4 working principles

　　2. Emergency command system and responsibilities

　　2.1 emergency organization

　　2.2 responsibilities of emergency organization

　　2.3 Expert Group

　　2.4 on-site organization

　　3. Monitoring, early warning and information reporting

　　3.1 Monitoring and early warning

　　3.2 Information report

　　4. Emergency response

　　4.1 Event classification

　　4.2 Graded response

　　4.3 Emergency treatment

　　4.4 Information release

　　4.5 End of emergency

　　Step 5 deal with the aftermath

　　5.1 aftermath disposal

　　5.2 Investigation and evaluation

　　6. Emergency support

　　6.1 Team Support

　　6.2 Financial guarantee

　　6.3 Communication Guarantee

　　6.4 Material and technical support

　　7. Supervision and management

　　7.1 Publicity, training and drills

　　7.2 accountability

　　8. supplementary provisions

　　8.1 Plan Management and Update

　　8.2 Plan formulation and implementation

　　1. General principles

　　1.1 Purpose of compilation

　　Establish and improve the province’s emergency response mechanism for water emergencies, quickly, efficiently and orderly deal with water emergencies, minimize casualties and property losses, ensure safe and smooth water traffic, and maintain social stability.

　　1.2 Compilation basis

　　People’s Republic of China (PRC) Emergency Response Law, People’s Republic of China (PRC) Safety Production Law, People’s Republic of China (PRC) Inland River Traffic Safety Management Regulations, National Maritime Search and Rescue Emergency Plan, Hunan Province Emergency Overall Emergency Plan and other laws, regulations and relevant provisions.

　　1.3 Scope of application

　　This plan is applicable to the prevention and emergency search and rescue of emergencies in the field of transportation within the administrative area of this province (including navigable waters under the jurisdiction of our province and closed waters with operating ships).

　　1.4 working principles

　　The emergency handling of water emergencies follows the principles of people-oriented, prevention-oriented, unified leadership, graded responsibility, territorial-oriented, compartmentalized, standardized according to law and scientific decision-making.

　　2. Emergency command system and responsibilities

　　The people’s governments of provinces, cities, prefectures, counties and cities shall set up emergency command institutions for water search and rescue, and be responsible for the emergency handling of water emergencies within their respective administrative areas.

　　2.1 emergency organization

　　The provincial people’s government set up the emergency headquarters (hereinafter referred to as the provincial headquarters) for water search and rescue in Hunan Province, with the deputy governor in charge of the provincial people’s government as the commander, the deputy secretary-general of the provincial people’s government, the director of the provincial department of transportation, and the city governor of the people’s government of the city where the incident occurred as the deputy commander. Provincial local maritime bureau, Provincial Water Resources Department, Provincial Government Information Office, Provincial Development and Reform Commission, Provincial Safety Supervision Bureau, Provincial Public Security Department, Provincial Health and Family Planning Commission, Provincial Agriculture Committee, Provincial Water Resources Department, Provincial Civil Affairs Department, Provincial Environmental Protection Department, Provincial Press, Publication, Radio, Film and Television Bureau, Provincial Finance Department, Provincial Meteorological Bureau, Provincial Communications Administration, Civil Aviation Hunan Supervision Bureau, Provincial Military Command, Hunan Armed Police Corps, Provincial Fire Corps (Provincial Emergency Rescue Corps) and other units are members.

　　The provincial headquarters office is located in the provincial local maritime bureau, and the director of the provincial local maritime bureau concurrently serves as the director of the office.

　　2.2 responsibilities of emergency organization

　　2.2.1 Provincial Headquarters

　　Unified command and coordination of the province’s water emergency response and search and rescue operations; Organize emergency search and rescue operations, mobilize the required manpower and material resources to do related work; Make decisions and give instructions on emergency handling and search and rescue operations of water emergencies; Ask for help and support from the state and the water search and rescue headquarters of the brother provinces, autonomous regions and municipalities as appropriate.

　　2.2.2 Provincial Headquarters Office

　　Implement the arrangements of the provincial headquarters, and formulate the working procedures and working rules for emergency handling and search and rescue operations on water; Check and urge the relevant departments (units) to implement emergency measures; Record, report, report and summarize information related to water emergencies; To study and coordinate the specific problems of emergency handling of water emergencies, and take the lead in drafting the summary and evaluation report of water emergencies; Complete other tasks assigned by the provincial headquarters.

　　2.2.3 Member units of provincial headquarters

　　The Provincial Department of Transportation is responsible for the organization, command and coordination of emergency response and search and rescue operations, and provides necessary search and rescue resources and technical support; Responsible for coordinating professional and social rescue forces to participate in water search and rescue; Guide the work of the provincial headquarters office.

　　The provincial local maritime safety administration undertakes the daily organization, command and coordination of water emergencies; Call other operating ships and social rescue forces in the accident waters to participate in search and rescue; To undertake the daily work of the provincial headquarters office; Organize and coordinate emergency rescue work.

　　The Provincial Water Resources Department provides real-time hydrological data for emergency response, organizes water level dispatching in the incident basin, and participates in the organization and coordination of emergency response on water.

　　The provincial government information office is responsible for coordinating the information release and public opinion guidance of water emergencies.

　　Provincial Development and Reform Commission guides and deliberates the preparation of water accident prevention planning and supports the construction of water accident prevention projects; Formulate emergency relief material supply guarantee plan.

　　Provincial Safety Supervision Bureau participates in the organization, command and coordination of water search and rescue and emergency disposal of large-scale polluted waters by ships.

　　The Provincial Public Security Department organized public security marine police and boats to assist traffic, maritime and professional emergency rescue teams to carry out water search and rescue emergency operations; Responsible for traffic control and public order maintenance of major emergency search and rescue activities.

　　The Provincial Health and Family Planning Commission is responsible for coordinating the emergency medical rescue work for water emergencies.

　　The Provincial Agriculture Committee is responsible for providing fishing boats with distress information and technical support needed for water search and rescue; Responsible for organizing fishing boats near the scene of the accident to participate in water search and rescue emergency operations.

　　The Provincial Civil Affairs Department participates in the social assistance and aftermath of water emergencies.

　　The Provincial Environmental Protection Department participates in the organization, command, coordination and pollution disposal of emergency disposal of large-scale polluted waters by ships, and is responsible for providing environmental monitoring information of polluted waters and environmental protection technical support related to water pollution.

　　Provincial Press, Publication, Radio, Film and Television Bureau organizes and coordinates radio, television and radio stations to release information in a timely manner.

　　The Provincial Department of Finance is responsible for timely application, arrangement and disbursement of search and rescue funds and materials, and cooperate with relevant departments to supervise their use.

　　The Provincial Meteorological Bureau is responsible for meteorological information and technical support for water search and rescue.

　　The Provincial Communications Administration is responsible for the communication support for emergency rescue operations on water.

　　Hunan Civil Aviation Supervision Bureau is responsible for providing civil aircraft distress information and search and rescue technical support, and participating in the command and coordination of civil aircraft search and rescue.

　　The provincial military command is responsible for organizing and coordinating the troops stationed in Hunan and the militia reserve forces to participate in water search and rescue and emergency disposal of large-scale polluted waters by ships.

　　Hunan Armed Police Corps is responsible for organizing and directing the Armed Police Force to participate in the emergency response of water emergencies.

　　Provincial Fire Corps (Provincial Emergency Rescue Corps) is responsible for organizing and directing fire forces to participate in the emergency treatment of water emergencies.

　　2.3 Expert Group

　　The provincial headquarters set up an emergency expert group for water emergency search and rescue, which is responsible for providing decision-making consultation and technical support for the provincial headquarters.

　　2.4 on-site organization

　　2.4.1 Field headquarters

　　After the water emergency, the people’s government of the place where the incident occurred quickly set up the on-site command, and under the leadership of the provincial command, directed and coordinated the on-site emergency response work of water search and rescue. The on-site headquarters is located in the local maritime safety administration, and the person in charge of the local people’s government is the commander.

　　The main responsibility of the on-site command is to carry out the task of water search and rescue issued by the provincial command; Be responsible for putting forward specific measures and plans for emergency treatment; Carry out various on-site emergency disposal work.

　　2.4.2 Emergency Rescue Working Group

　　According to the needs of emergency response, the field command has several working groups.

　　(1) Information processing group: carry out the instructions of the headquarters, be responsible for the organization, command and coordination of the emergency search and rescue work site, and designate a command ship.

　　(2) Evaluation and judgment group: make a preliminary evaluation according to the development of on-site danger and the implementation effect of emergency response plan, and put forward opinions on adjusting emergency response measures or suggestions on ending emergency response actions.

　　(3) Logistics Support Group: guiding ships and facilities to organize self-help and mutual rescue; Responsible for mobilizing social assistance forces to participate in emergency rescue.

　　(4) Emergency Action Team: When the dangerous situation changes sharply, it has the right to take relevant reasonable actions on the premise of ensuring to reduce casualties and economic losses of the accident and reduce water pollution.

　　(5) Monitoring and monitoring group: responsible for monitoring and monitoring the dangerous situation at the emergency search and rescue site, reporting the situation at the site or asking for emergency matters.

　　(6) Security guard group: responsible for on-site traffic control and maintaining navigation order.

　　(7) Aftercare team: responsible for organizing, coordinating and directing the work of clearing and evacuating the site, and doing a good job in aftercare.

　　3. Monitoring, early warning and information reporting

　　3.1 Monitoring and early warning

　　Water search and rescue agencies at all levels should strengthen the investigation, analysis and registration of dangerous sources and dangerous areas that may cause water emergencies in the waters under their jurisdiction in combination with daily management, organize risk assessment, determine the risk level, and timely notify the local people’s government. Relevant units should formulate preventive measures and strengthen daily monitoring and inspection according to the hazard sources, risk levels and nature of water emergencies.

　　Early warning information monitoring departments shall, according to their respective responsibilities, release early warning information of meteorological, hydrological and geological disasters to relevant parties through information broadcasting channels. Early warning information generally includes meteorological, hydrological, geological and other natural disaster forecasting information and other information that may trigger water emergencies.

　　3.2 Information report

　　3.2.1 Reporting procedure

　　When the personnel on duty find a dangerous situation or receive an incident report, they should immediately contact the relevant departments at the place where the incident occurred, quickly confirm the nature and grade of the water emergency, and report to the superior emergency agency in time.

　　3.2.2 Report content

　　The report of water emergency information should be fast and accurate, including the following contents:

　　(1) the time and place of the incident;

　　(2) the number of casualties, economic losses, damage degree, etc.;

　　(3) Relevant information about the ship and its subordinate units;

　　(4) The brief course of the incident and the current disposal situation;

　　(5) Other relevant matters that need to be reported.

　　4. Emergency response

　　4.1 Event classification

　　According to the degree of harm to life safety and water environment and the development trend, water emergencies are divided into four levels: general (IV), major (III), major (II) and particularly major (I).

　　4.1.1 A general water emergency (Grade IV) meets one of the following conditions: less than 3 people fall into the water to be rescued or less than 10 people are in distress; Accidents (hereinafter referred to as accidents) such as collision, hitting rocks, fire, contact damage, grounding and windstorm of ordinary cargo ships below 300 gross tons pose a threat to the life safety of ships and personnel; The oil spill from the ship is less than 5 tons.

　　4.1.2 A major water emergency (Grade III) meets one of the following conditions: more than 3 people and less than 10 people fall into the water to be rescued, or more than 10 people and less than 30 people are in distress; Accidents of ordinary cargo ships with a gross tonnage of more than 300 tons and a gross tonnage of less than 1,000 tons pose a threat to the life safety of ships and personnel; The oil spill from the ship is more than 5 tons and less than 20 tons.

　　4.1.3 A major water emergency (Grade II) meets one of the following conditions: more than 10 people and less than 30 people fall into the water to be rescued, or more than 30 people and less than 60 people are in distress; Accidents of ordinary cargo ships with a gross tonnage of more than 1,000 tons and less than 3,000 tons pose a threat to the life safety of ships and personnel; The oil spill from the ship is more than 20 tons and less than 100 tons.

　　4.1.4 A particularly serious water emergency (Grade I) meets one of the following conditions: more than 30 people fall into the water to be rescued, or more than 60 people are in distress; Accidents of ships with a gross tonnage of more than 3,000 tons pose a threat to the life safety of ships and personnel; The ship spilled more than 100 tons of oil.

　　In the above quantitative expressions, "above" includes the number, and "below" does not include the number.

　　4.2 Graded response

　　4.2.1 Level IV response

　　In the event of a general water emergency, the county-level water search and rescue emergency headquarters immediately launched a level IV response, organized the implementation of emergency search and rescue, and quickly reported the relevant situation to the city-level water search and rescue emergency headquarters.

　　4.2.2 ш level response

　　In the event of a major water emergency, the city and state water search and rescue emergency headquarters immediately launched a class ш response, organized the implementation of emergency search and rescue, and quickly reported the relevant situation to the provincial headquarters.

　　4.2.3 Level II response

　　In case of a major water emergency, the provincial headquarters immediately launched a Class II response, and each member unit quickly carried out emergency search and rescue operations according to the instructions of the provincial headquarters and the responsibilities specified in the plan, and timely reported the relevant information to the National Maritime Search and Rescue emergency headquarters.

　　4.2.4 Level I response

　　In case of particularly serious water emergencies, the provincial headquarters shall report the development of water emergencies and the emergency situation of search and rescue to the provincial people’s government and the National Maritime Search and Rescue Center in a timely manner, and draw the National Maritime Search and Rescue Center to start a Class I response, and the provincial headquarters shall conduct emergency search and rescue under the guidance of the National Maritime Search and Rescue emergency headquarters.

　　4.3 Emergency treatment

　　After the dangerous situation occurs, the emergency command organization of water search and rescue at the incident site should immediately take measures to control the development of the situation, organize the pre-disposal work such as search and rescue, and report the development of the situation in a timely manner according to regulations. The relevant person in charge of the local people’s government immediately rushed to the scene, set up an on-site command organization, organized and directed the departments of transportation, maritime affairs, public security, fire protection, medical and health care to carry out emergency response, and took the following measures according to the actual situation:

　　(1) according to the specific location or possible location of the search and rescue target, determine the search and rescue area.

　　(2) To formulate the specific rescue plan on site and organize its implementation, and implement it after it is approved by the superior headquarters if necessary.

　　(3) According to the actual situation on site, several emergency response teams were set up to provide rescue.

　　(4) Organize and direct rescue forces at or near the scene of the incident, designate appropriate search and rescue methods for the rescue forces at the scene, assign search and rescue tasks, organize search and rescue operations, and ensure their safety at the same time.

　　(5) Quickly organize on-site treatment and transfer of the wounded.

　　(6) Fully understand and master the site situation, determine the site contact information, keep in touch with the superior headquarters, and feed back the site information in time.

　　(7) Make detailed records of on-site search and rescue operations, including on-site rescue forces and equipment, accidents or dangerous situations, search and rescue, etc.

　　(8) In case of pollution, sampling and evidence collection shall be organized and confirmed by the parties concerned.

　　(9) According to the change of site conditions, determine the use plan of rescue forces, and make suggestions on the emergency rescue plan formulated by the superior headquarters.

　　(10) Responsible for maintaining the order at the scene and dealing with the aftermath.

　　4.4 Information release

　　Water search and rescue emergency command institutions at all levels shall, in accordance with the relevant provisions of the Emergency Plan for Emergency News Release in Hunan Province, do a good job in information release.

　　4.5 End of emergency

　　After ships, facilities, aircraft or personnel are out of danger or pollutants have been effectively controlled and removed, the emergency response is declared to be over according to the principle of who started and who terminated.

　　Step 5 deal with the aftermath

　　5.1 aftermath disposal

　　The local medical and health department is responsible for the rescue of the injured; The local civil affairs department is responsible for the resettlement and rehabilitation of the rescued and dead, and organizes social assistance; The Hong Kong, Macao and Taiwan Affairs and Overseas Chinese Affairs Department of the local people’s government is responsible for the resettlement of Hong Kong, Macao and Taiwan nationals and foreign casualties.

　　5.2 Investigation and evaluation

　　After the rescue and disposal of water emergencies, the office of the water search and rescue emergency command organization shall, jointly with the member units of the command organization and the people’s government at the place where the incident occurred, investigate and summarize and evaluate the incident, and submit the summary and evaluation report to the people’s government at the same level and the superior water search and rescue emergency command organization.

　　6. Emergency support

　　6.1 Team Support

　　The professional teams of city and state water search and rescue and the water forces stationed in Hunan are the pre-disposal teams of water search and rescue emergency operations, and the professional emergency search and rescue teams of provincial water emergency search and rescue bases are the follow-up disposal teams, and request reinforcements to the National Maritime Search and Rescue Center and the water search and rescue emergency command institutions of neighboring provinces, autonomous regions and municipalities as appropriate.

　　6.2 Financial guarantee

　　The people’s governments at or above the county level shall include the emergency funds for water search and rescue in the financial budget.

　　6.3 Communication Guarantee

　　The offices of water search and rescue emergency command institutions at all levels shall establish an emergency address book for water emergencies, clarify the contact information, and strengthen the contact between member units and relevant departments and units.

　　6.4 Material and technical support

　　People’s governments at all levels and their relevant departments shall do a good job in emergency material reserve according to relevant laws and regulations, establish and improve the emergency material reserve network, allocate and update the emergency distribution system, and ensure the materials needed for emergency; Establish emergency equipment type, quantity, performance, storage location and standby system; Organize relevant scientific research institutions to carry out technical research on the development trend of water emergencies in our province.

　　7. Supervision and management

　　7.1 Publicity, training and drills

　　People’s governments at all levels, transportation departments and water emergency search and rescue headquarters should increase the publicity of emergency laws and regulations on water emergencies, prevention of hedging, self-help and mutual rescue, and strengthen the business training of staff of water search and rescue institutions and professional rescue forces on water search and rescue, so that they can master the knowledge and skills of water emergency search and rescue.

　　Emergency command agencies for water search and rescue at all levels should organize emergency drills regularly or irregularly, further clarify the responsibilities of each post in emergency response, and timely revise and improve the plans in view of the problems and deficiencies in the plans.

　　7.2 accountability

　　Units and individuals who do not perform their duties conscientiously, neglect their duties and cause serious losses shall be given corresponding punishment according to law; Those who violate the law shall be investigated for legal responsibility according to law.

　　8. supplementary provisions

　　8.1 Plan Management and Update

　　According to the actual situation, the Provincial Department of Transportation promptly submitted to the provincial people’s government to revise and improve this plan.

　　8.2 Plan formulation and implementation

　　This plan shall come into force as of the date of promulgation.

　　As early as many years ago, in order to make up for the shortcomings of Toyota’s high-end brand Lexus in compact models, CT200h was introduced to enhance its overall competitiveness. However, according to the sales data, from the launch of the CT200h to the first quarter of this year, the global cumulative sales volume is only about 267,000 vehicles. In this big market of China, the sales volume of the CT200h accounts for less than 10% of the total sales volume of Lexus. It can be seen that this entry-level car, which has been launched for nearly six years, has not brought surprises to Lexus.

　　▲ The sales volume is not satisfactory, and the production of CT200h is discontinued.

　　Perhaps the sales volume is not satisfactory. According to reliable sources, the CT200h will face production suspension, and Alain Uyrrenhoven, president of Lexus Europe, revealed that "the CT200h will be replaced by a hybrid small SUV". Of course, if we study why the CT200h stopped production according to the meaning of Lexus, we may only think that the SUV market is overheated and the cars are not popular, but is this really the case?

　　As we all know, compared with British finidi and Acura, Lexus, as a member of Japanese luxury Three Musketeers, has performed strongly in the market with its excellent quality and good service. Therefore, when the compact car CT200h, which started at more than 200,000 yuan, was launched, many people began to worry that it would more or less destroy the long-standing good image of Lexus.

　　▲ Hidden quality hazards affect the brand image, or become the main reason for the shutdown of CT200h.

　　Because it is a hybrid of oil and electricity, CT200h is excellent in fuel economy, and its bold design also shows the charm of Lexus. However, in details, CT200h really proves the truth of "a penny gets a share". First of all, in terms of materials, CT200h’s materials are very general, the suspension is hard and the comfort is average.

　　A criticism that is particularly worth mentioning is the sound insulation effect of CT200h h. It has been reported by the media that when the speed reaches above 80km/h, the noise inside the CT200h is as high as 79dB(70-90 dB is noisy and nerve cells are destroyed), which has seriously interfered with the driver’s driving and the safety hazard is very obvious.

　　In addition, a friend of Xiaobian’s car owner said that the CT200h he was driving had many problems, especially the abnormal brake noise. When Xiaobian searched online for the abnormal brake noise of CT200h, there was almost a buzz on the Internet. What’s more, some car owners couldn’t wait to show the abnormal brake noise in less than three or four days, which was really a headache.

　　Therefore, Xiao Bian guessed that the CT200h had the idea of stopping production because of its poor sound insulation effect and abnormal sound, which was not conducive to the establishment of a good brand image.

　　▲ frequent and numerous recalls, but ineffective and negative?

　　Originally, I wanted to fill the gap in the compact car market. Clinker CT200h, an entry-level model, did not give force, did not bring surprises to Lexus, and more or less brought some panic. So, is it the best choice to stop production of CT200h to reverse Gankun and re-establish the brand image?

　　In fact, in view of the defects of vehicles, car companies will start a recall plan to improve the problems of vehicles. Although Lexus has not implemented a recall for abnormal noise, Lexus has started a recall plan before because of the hidden dangers of fuel leakage and airbags, involving 37,423 vehicles, including CT200h.

　　The number of recalls is amazing, but this is not the problem of this recall. Xiaobian asked the owner’s friend. When he told him that his CT200h was going to be recalled for inspection and maintenance, he said he didn’t know. When he called the local 4S shop, the staff of the 4S shop admitted that his vehicle needed to be recalled. In other words, even if Lexus started the recall plan, it did not take the initiative to contact the owner for inspection and maintenance, but the owner reflected that the 4S shop admitted it. Does such a poor and negative service attitude really come from Lexus?

　　Coincidentally, another incident deserves our attention. A car owner once made a complaint on the car complaint network. He said that his newly bought 2014 lexus ES300 had just driven for 200 kilometers when the ABS fault light called the police, and the brake pedal didn’t respond at all. He was asked to replace the ABS controller assembly after going to the 4S shop for inspection.

　　The description of this problem coincides with the reason stated in Lexus’ recall regulations in April this year. The recall was as follows: due to the manufacturing reasons of a specific supplier, the oil pressure required for braking anti-lock braking system (ABS) and body stability control system (VSC) may not be provided normally, which may affect the driving stability of the vehicle and have potential safety hazards, and a total of 15,322 lexus ES200, ES250 and RX200t vehicles were recalled.

　　Putting the owner’s complaint together with the Lexus recall, we found that the owner’s ES300 also had the problems mentioned in the recall, but the ES300 was not within the scope of the recall!

　　Summary:Now, due to various quality problems, the CT200h is already in water under the bridge, and it has to stop production on the pretext of "good SUV market prospects". Apart from the tragic product CT200h, the frequent recalls of Lexus this year and the large number of them are really unsightly, but the negative and ineffective behaviors such as not notifying the owners in time and incomplete recalls are the facts that it is difficult to cover up when CT200h stops production.

This year, all Tibetan counties in our province will be stripped of their hats, and there will be four counties in Liangshan Prefecture, including Leibo, Ganluo, Yanyuan and Muli. Deep poverty-stricken areas such as Tibetan areas and Yi areas will be the focus of assessment and evaluation of poverty alleviation. The picture shows Moshi Village, Paha Township, Leibo County. A beautiful picture of a new village and a new village is slowly unfolding. The villagers have lived in safe housing and are working hard to get rid of poverty. (Reporter   Xiao Yuyang)(Visual Sichuan data picture)

interpret

Recently, in 2019, a video conference on poverty exit acceptance and assessment was held in Chengdu, and arrangements were made for the deployment of poverty alleviation assessment and dynamic management of poverty alleviation targets in our province this year.

At the meeting, the deputy secretary-general of the provincial government, the director of the provincial poverty alleviation office and the director of the provincial poverty alleviation and development bureau stressed at the beginning that the rest of this year and next are hard bones, and the poverty level is deeper and it is more difficult to get rid of poverty, especially in 2019. It is also the highest peak of poverty-stricken counties, and the difficulty, density and intensity of work are unprecedented.

In this context, doing a good job in the assessment of poverty alleviation this year and improving the management of poverty alleviation targets will lay a solid foundation for our province to finally win the battle against poverty in an all-round way.

Look at the changes

Focusing on deep poverty areas and the quality of poverty alleviation, different "candidates" and "test sites" are different.

If the assessment is compared to an exam, what are the "test sites"?

The relevant person in charge of the Provincial Poverty Alleviation and Development Bureau introduced that it mainly focuses on "two guarantees and three guarantees", focusing on deep poverty-stricken areas, focusing on the quality of poverty alleviation, and focusing on "four non-picks".

The person in charge said that the above work not only pays attention to key areas, such as deep poverty areas, but also emphasizes key tasks, that is, implementing the "two guarantees and three guarantees", while paying attention to groups that have been lifted out of poverty and giving full consideration to all aspects of poverty alleviation.

According to different "candidates", the focus of the exam is also different.

Although the detailed assessment plan has not yet been released, according to the assessment work arrangements in previous years, there are roughly the following aspects.

For counties with lighter poverty alleviation tasks, focus on assessing the assistance of the remaining poor, improving the long-term poverty alleviation mechanism and consolidating and improving the results of poverty alleviation; Counties that have taken off their hats should also assess the implementation of the "four don’t take off" (taking off their hats without responsibility, taking off their hats without assistance, taking off their hats without policy, and taking off their hats without supervision).

Counties with heavy poverty alleviation tasks will focus on assessing the completion of poverty alleviation tasks and the implementation of poverty alleviation responsibilities, policy implementation and work implementation; In counties that have not taken off their hats, it is also necessary to focus on assessing the progress of poverty reduction, the quality of poverty alleviation and the completion of the bottom line tasks.

From June to September this year, our province carried out the "two worries and three guarantees" to look back at the big investigation work, and required full coverage of all poor households who set up files. It was the first province in China to carry out the "two worries and three guarantees" full coverage investigation.

Paying attention to the rectification of major investigation problems and data quality is also the focus of this year’s assessment and evaluation work. At the beginning of the reduction, it is necessary to assess whether the major investigation is strictly based on the facts to find problems and whether it is a benchmark for rectification.

Seeking precision

Do a good job in dynamic management of poverty alleviation objects to ensure the authenticity of data.

Precision is the key to getting rid of poverty. At present, it is approaching the final stage of poverty alleviation, and the dynamic management of poverty alleviation targets, striving for precision and precision, is the focus of the next stage of our province.

"The dynamic adjustment and labeling of poverty alleviation targets mainly completes poverty alleviation of poor households, withdrawal of poor villages, natural increase and natural decrease of family members of poor households (including poverty-stricken households)." According to the beginning of the year, the collection, updating and entry of information on poverty alleviation targets are mainly the collection and entry of basic information of family members of newly-added poor households, the updating of basic information of poor households (including poverty-stricken households) and poverty-stricken villages (including exit villages), and the verification, collection and entry of information on poverty-stricken households (including poverty-stricken households over the years).

It is necessary not only to adjust in time, but also to ensure accuracy. Data quality is the lifeline of establishing a card, which not only affects the analysis, judgment and macro-decision of the situation of poverty alleviation, but also affects the quality of poverty alleviation.

According to the relevant person in charge of the Provincial Bureau of Poverty Alleviation and Development, next year, the state will organize a census of all poverty-stricken counties, and the basic basis of the census is to file data, so it is extremely urgent to completely solve the data quality problem.

The meeting proposed that problems such as inaccuracy and inaccuracy found in major investigations should be actively rectified and revised in the national system; Print the basic information of poor households and the comparison table of assistance measures through the information system, carry out information collection at home, and verify and approve the data of the filing card; If it needs to be revised after verification and approval, it should be improved in the information system.

"The directors of poverty alleviation offices in cities, counties and cities should take the verification and approval (dynamic adjustment) of poverty alleviation data as the’ first priority’ in their daily work." At the beginning of the year, it was emphasized that when talking about the problems existing in poverty alleviation, we should use the results of "big investigation" in a unified way and resolutely put an end to the phenomenon of "one set of data for one department". "All counties and cities should pay attention to data quality and make the data consistent and logical." (Reporter   Houchong)

microblog comment

The "key year" should be strictly tested.

2019 is a very crucial year. This year’s work has been done, and next year’s poverty alleviation will have a more solid foundation.

How to ensure that this year’s work is in place? Assessment is an important link.

Is the "two guarantees and three guarantees" in place? What is the effect of industrial development? How is the assistance work going? Have poor households, poor villages and poor counties reached the standard of poverty alleviation? Is poverty exit in line with the procedure? This series of question marks need to be straightened in the assessment.

Looking back on the assessment of poverty alleviation in our province in the past two years, the careful work of investigators is impressive. Some investigators go straight to the kitchen after entering the house to see if there is safe drinking water; Some repeatedly check the data in the poverty alleviation manual; If the poor people are not at home, the investigators will try their best to contact each other for telephone verification.

It can be said that the more we get rid of poverty, the more we face "hard bones", and we need to strictly assess and evaluate the work, so as to really "feel the pulse" for getting rid of poverty. Only in this way can we have a bottom in our hearts and ensure that Sichuan and the whole country will build a well-off society in an all-round way at the end of next year. (Luan xiaojing)

The Outline of New World History, edited by Qian Chengdan, a professor of Peking University History Department, is the final result of a major special research project of humanities and social sciences of the Ministry of Education. This project aims to explore China’s knowledge system of world history and build a framework for writing China-style world history textbooks. "Outline" is equivalent to the curriculum standard, which provides ideas, expands the connotation on the basis of "Outline", and can form a rich and comprehensive knowledge system of world history, thus spreading the correct view of world history to Chinese people and providing complete knowledge of world history. This article is taken from the Outline of New World History and reprinted by The Paper with authorization.

With the arrival of the mercantilist period, the expansion process of the west began. Mercantilism theory holds that precious metals are the embodiment of wealth, so seeking gold and silver overseas has become the main policy goal of western European governments. They encouraged their nationals to explore the sea, to explore overseas trade and to rob colonies. Colonies are of special significance to the mercantilist policy. Colonies bring markets as well as commercial profits, gold and silver to the home country.

Portuguese navigators first entered the Atlantic Ocean, and they opened a sea passage from Europe to eastern Asia in a century. In 1415, the Portuguese captured the Arab city of Ceuta in northern Morocco, which marked the beginning of European exploration and control of overseas territories. In the 15th century, most of the gold that arrived in Europe came from West Africa and the Ashanti black community near Ghana today. Muslim merchants brought gold from Africa and then took it north across the Sahara to Mediterranean ports. Portugal intervened in this gold business by force. Under the rule of King Joao II (reigned from 1481 to 1495), the Portuguese established trading ports and border trading posts on the coast of Guinea, and entered the mainland directly to Timbuktu. Portuguese ships arrived in Lisbon loaded with gold and controlled the circulation of gold in Europe by 1500.

Portugal established more than 50 trading ports between Africa and East Asia, and established the earliest commercial port empire. In San Giorgio da Mina, they engaged in the slave trade in West Africa; In Mozambique, they tried to control the gold trade in South Africa; In the Strait of Hormuz, they controlled the entrance to the Persian Gulf; In Goa, they sell Indian pepper; In Malacca, they supervise cargo ships from the South China Sea of China to the Indian Ocean; Through Ternat Island, they also controlled the clove and nutmeg trade in Maluku spice islands. Alfonso de Abuquiki, commander of the 16th century Portuguese Indian Ocean Corps, led the fleet to capture Hormuz in 1508, Goa in 1510 and Malacca in 1511, thus controlling the trade in the Indian Ocean, forcing all merchant ships to buy safety passes before they could dock at Portuguese trading ports. Ships without passes will be confiscated together with the goods. Like Portugal, British and Dutch businessmen also set up commercial ports on the coast of Asia, seeking to establish trade routes through commercial ports, but they have not yet thought of controlling freight on the high seas. In 1565, the Spanish army came to the Philippine Islands and named it after King Philip II of Spain. During 1603-1819, Spanish colonists had six large-scale conflicts with Filipinos and China businessmen in Manila, killing thousands of China businessmen.

The original purpose of European navigation was to find a new route to the East. Columbus, an Italian navigator, loved sailing adventures since he was a child. He read The Travels of Marco Polo and longed for India and China. At that time, the theory of the earth circle was very popular, and Columbus believed it. He successively asked the kings of Portugal, Spain, England, France and other countries for funding to realize his plan of sailing westward to the eastern countries, but all of them were rejected. Finally, he persuaded the Spanish king on the grounds of oriental products such as silk, porcelain, tea and spices, and was able to make it. In 1492, under the orders of Spanish King Isabella and Ferdinand, Columbus took the credentials to Emperor China, led three ships and 87 sailors to sail from Barros Port and crossed the Atlantic Ocean, but ended up in Bahamas, Cuba and Haiti. In the next three voyages, I visited Jamaica, Puerto Rico and the coastal areas of the American continent. He crossed the Atlantic four times and "discovered" America, becoming a famous navigator in history. The Spanish immediately entered these areas for early colonization. They conquered South America, robbed local wealth, slaughtered local residents, and sent a large number of blacks from West Africa to America to engage in slave labor, thus developing America. These activities quickly wiped out the Indians in South America. When Columbus came to Hispaniola in 1493, the population there was nearly 100,000. By 1570, there were only 300 people left.

In 1519, the navigator Ferdinand Magellan (1480-1521) sailed westward, hoping to find a route to the southeast coast of Asia. Magellan traveled from San Roca to Brazil, passed through the strait between the South American continent and Tierra del Fuego, and crossed the Pacific Ocean, Guam and the Philippines in 1521. He was killed by local residents for interfering with the infighting on the island. After Magellan’s death, the voyage continued. In 1522, the Victoria returned to Spain via the Indian Ocean, the Cape of Good Hope and the Atlantic Ocean, completing the first voyage around the earth in human history. This voyage verified the theory that the earth is spherical and brought back a huge amount of information about the Pacific Ocean.

The above-mentioned exploration activities triggered the competition between Portugal and Spain for overseas colonies. In 1494, Spain and Portugal signed the Tolde-silas Agreement, which demarcated the north-south boundary 370 leagues west of Cape Verde Islands. It was stipulated that Spain’s sphere of influence was to the west and Portugal’s sphere of influence was to the east, which was the first time that western countries divided up the world.

Colonization opened the way for primitive accumulation of capital in Europe. According to statistics, from 1493 to 1600, the Portuguese plundered more than 270,000 kilograms of gold in Africa alone. From 1521 to 1600, Spain plundered more than 200,000 kilograms of gold and 18 million kilograms of silver from America. Most of the capital in western Europe comes from gold and silver in America, which is related to Spain’s overseas colonization and monopoly of trade with the East to some extent. During 1519-1521, Spain conquered Mexico and Peru, began to plunder gold and silver there in 1531, and began to organize mining in America with new technology in 1540. In the trade with the East, the king of Spain levied a 20% trade tax, so Spain gained huge wealth from overseas trade. However, Spain did not keep this wealth. On the contrary, gold and silver flowed into other European countries, which promoted the development of capitalism in other regions.

Later, British and Dutch businessmen also came to Asia to set up commercial ports on the coastline and establish maritime trade lines through these commercial ports. The activities of the colonists promoted commercial development and established a global trading system in a real sense. Before that, the world’s commercial trade was regional, but the expansion of European countries at sea connected the world in the same trading system. The products made in Europe crossed the Atlantic Ocean westward in exchange for silver from Mexico, minerals from Peru and agricultural products such as sucrose and tobacco in the Caribbean. European textiles, guns and other manufactured goods went south to West Africa in exchange for African slaves and then transported them to work in tropical and subtropical plantations in the Western Hemisphere. China’s silk and porcelain are directly transported to Europe, and are warmly welcomed by the courts and upper classes of various countries; Tea and spices were shipped to Europe and America and eventually became mass consumer goods. Through the establishment of the global trading system, "the transformation from history to world history" has begun.

In early modern times, the trade routes in Europe were basically the same as those in the previous two centuries. Northern Italy and Flanders are the most developed cities and industrial centers in Europe, and they are both suppliers of woolen clothes in the European market. Northern Italy is also a producer of silk and other valuable clothing materials, while Flanders produces linen, ribbons and carpets. These two regions also have advanced shipbuilding and metal processing industries. Venice monopolizes the eastern trade through the eastern Mediterranean countries and islands. The goods from the east are mainly spices and other luxury goods, which are sold in Europe by Italian and German businessmen. They can go southwest to France and Spain and northeast to Germany and Baltic countries via the Italy-Flanders axis. The British route on the Italian-Flemish axis is mainly responsible for transporting the semi-finished clothes from Britain to Flanders and brabant for processing.

Gold and silver from America entered the northwest of Europe through Spain, making these places eventually become the most developed economic centers in Europe, including low-lying countries, northwest France and southeast Britain. The "lowlands" used to be the commercial meeting place between Italy and the Hanseatic League, but Spain’s tight financial situation and huge trade deficit made businessmen and bankers in the lowland countries, Italy and Germany become the actual leaders of international trade. The low-lying countries were the bridge between the North and the South in the Middle Ages, so Antwerp became the trade center of Spain, Portugal and Germany. In the 16th century, Antwerp was the largest international financial market in Europe, and every move here affected the whole European economy. The delay of Spanish and Portuguese cargo ships can cause commercial chaos in Antwerp, and even lead to bank failures in augsburg and Ulm. After gaining independence, the Netherlands followed Portugal and Spain and rapidly expanded its overseas trade. It monopolized the spice trade and controlled the eastern routes by crowding out Portugal’s forces in the East through war. In the Atlantic, it can also squeeze into Spain’s sphere of influence and insert a foot in trade. The 17th century became the "century of Holland", and Holland was the "sea coachman" at that time. Britain and France also rapidly expanded overseas after the reunification of the country. They successively established colonies in America, Asia and Africa, and replaced the Netherlands in the second half of the 17th century, becoming the protagonists in the struggle for world commercial hegemony in the next century.

Several main statistics of the results of the second national population census

(1964)

　　 (1) Total population

　　　 At 24: 00 on June 30, 1964, the national population was 723,070,269. The population of 28 provinces, municipalities and autonomous regions (Tianjin was then affiliated to Hebei Province) and active servicemen was 694,581,759, including 356,517,011 men, accounting for 51.33%; There are 338,064,748 women, accounting for 48.67%. The population of Taiwan Province, Hong Kong and Macao compatriots and overseas Chinese is 28,488,510.

　　　 The population of each province, city and autonomous region is as follows: (unit: people)

　　　 Beijing 7,568,495
　　　 Hebei Province 45,687,781
　　　 Inner Mongolia Autonomous Region 12,348,638
　　　 Shanxi Province 18,015,067
　　　 Liaoning Province 26,946,200
　　　 Jilin Province 15,668,663
　　　 Heilongjiang Province 20,118,271
　　　 Shanghai 10,816,458
　　　 Jiangsu Province 44,504,608
　　　 Zhejiang Province 28,318,573
　　　 Anhui Province 31,241,657
　　　 Jiangxi Province 21,068,019
　　　 Fujian Province 16,757,223
　　　 Shandong Province 55,519,038
　　　 Guangdong Province 42,800,849
　　　 Guangxi Zhuang Autonomous Region 20,845,017
　　　 Hunan Province 37,182,286
　　　 Hubei Province 33,709,344
　　　 Henan Province 50,325,511
　　　 Sichuan Province 67,956,490
　　　 Yunnan Province 20,509,525
　　　 Guizhou Province 17,140,521
　　　 Xizang Autonomous Region 1,251,225
　　　 Shaanxi Province 20,766,915
　　　 Gansu Province 12,630,569
　　　 Qinghai Province 2,145,604
　　　 Xinjiang Uygur Autonomous Region 7,270,067

　　 (2) Population of all ethnic groups

　　　 On June 30, 1964, there were 651,296,368 Han people in 28 provinces, municipalities and autonomous regions, accounting for 94.22% of the total population. The population of ethnic minorities is 39,883,909, accounting for 5.78%.

　　　 Ethnic groups with a population of more than one million are: Mongolian 1,965,766; 4,473,147 Hui people: 2,501,174 Tibetans; 3,996,311 Uighurs; 2,782,088 Miao people; 3,380,960 Yi people; 8,386,140 Zhuang people; 1,348,055 Buyi people; 1,339,569 Koreans; There are 2,695,675 Manchu people.

　　 (3) Population age

　　　 On June 30, 1964, the working-age population in China (men aged 16 to 59 and women aged 16 to 54) was 341,491,424, accounting for 49.17% of the total population.

　　　 The population under the age of 1 to 14 is 280,671,035, accounting for 40.4% of the total population. Among them: 114,287,925 school-age children aged 7 to 12; There are 135,422,127 children under 6 years old. The population over 61 is 38,171,010, accounting for 5.5% of the total population. There are 4,900 people over 100 years old (2,134 men and 2,766 women). The highest age is 150 years old, and there is one person.

　　 (4) Education level of the population

　　　 On June 30, 1964, among the population of 28 provinces, municipalities and autonomous regions, there were 2,875,401 people with university education, 9,116,831 people with high school education, 32,346,788 people with junior high school education and 195,824,459 people with primary school education.

　

　　On February 27th, the Pterosaur Ⅱ UAV developed by aviation industry corporation of china Chengdu Aircraft Design and Research Institute successfully made its first flight, which not only has multiple characteristics such as high cost performance, long endurance and multi-purpose, but also is suitable for military, police and civilian fields.

　　All four engines of the large-scale amphibious aircraft AG600 (Jiaolong 600) for fire fighting and water rescue were successfully tested for the first time, which laid a solid foundation for the next first flight. The aircraft was equipped with a communication and navigation system developed by CLP Avionics Co., Ltd.

　　The deep connection and transformation between "people joining the army" and "military conversion to the people" has stimulated the vitality of enterprise development and innovative achievements have emerged continuously. As an important bearing area of national defense science and technology industry, Chengdu has formed a relatively complete industrial cluster in aviation, aerospace, information security, military electronics, nuclear energy and nuclear technology applications, electromechanical equipment and materials, and has strong industrial strength.

　　Based on its own advantages, Chengdu will further implement innovation-driven and integration of defense and civilian technologies, and cultivate and develop the national defense science and technology industry as a high-end growth industry. According to integration of defense and civilian technologies’s industrial development plan, Chengdu will vigorously develop integration of defense and civilian technologies industrial cluster park as the starting point, accelerate the reform and innovation of integration of defense and civilian technologies mechanism system, and open up the innovation channel of integration of defense and civilian technologies; We will focus on building a "1+N" industrial system in integration of defense and civilian technologies, and strive to cultivate 100 billion-level clusters by 2025.

　　Breaking institutional barriers and stimulating endogenous vitality of both military and civilian sides

　　Sichuan Province has been listed as one of the experimental areas to systematically promote comprehensive innovation and reform. To shoulder the heavy responsibility of being the first city in Sichuan, Chengdu must open up an innovation channel for deep integration of military and civilian, and set an example for accumulating experience for comprehensive innovation and reform.

　　In Chengdu Kaitian Electronics Co., Ltd., several young R&D personnel are preparing to start a business by using AVIC’s joint creation platform. The military technology based on Kaitian Electronics’ sensors for decades has set foot in the civil security industry, which is also a transformation growth point of Kaitian Electronics.

　　"The integration of military products and civilian products is very different, and it is necessary to reform the mechanism and system, and even many aspects such as product modeling and design concepts need to be changed." The relevant person in charge of Kaitian Electronics said that introducing strategic investors to form joint-stock companies and accelerating the reform of institutional mechanisms have also become one of the key directions of the company’s future efforts.

　　Breaking the shackles of institutional mechanisms and giving full play to the role of the market can stimulate the endogenous vitality of both the military and the people. "integration of defense and civilian technologies involves many fields, such as industry, science and technology, military affairs and education. It is a complex systematic project. There are different management systems and mechanisms between military and civilian enterprises, and institutional mechanisms and technologies cannot be well integrated." The relevant person in charge of the Municipal Economic and Information Committee also said that to promote the development of integration of defense and civilian technologies, it is necessary to fundamentally solve these problems.

　　Taking accelerating institutional innovation as a breakthrough, Chengdu focuses on key projects, focusing on screening major projects in integration of defense and civilian technologies with large investment, strong driving ability and leading technical level, and strengthening follow-up; Actively implement the strategic agreement with the military industry group and promote the signing of a number of projects; Through the project guidance, actively guide social resources to participate in the reform of mixed ownership of military industry, give full play to the advantages of flexible system and mechanism, rapid decision-making and strong financial strength of private enterprises, and explore the development model of integration of defense and civilian technologies in which civilian enterprises participate in the reorganization and restructuring of military enterprises by means of equity participation, holding and acquisition.

　　In order to accelerate the deep integration of military and civilian and promote the industrial development of integration of defense and civilian technologies, in the second half of last year, the "Thirteenth Five-Year Plan for the Industrial Development of integration of defense and civilian technologies in Chengdu" and "Several Policies and Measures for Chengdu to Accelerate the Industrial Development of integration of defense and civilian technologies" were successively issued, which clarified the development direction and put forward the goal of building a national integration of defense and civilian technologies Innovation Demonstration Zone, "by the end of the Thirteenth Five-Year Plan, the income from the main business of integration of defense and civilian technologies’s industry exceeded 150 billion yuan".

　　The support of relevant policies has stimulated the vitality of enterprise development: Sichuan Haite High-tech Co., Ltd. actively participated in the reform of mixed ownership of state-owned enterprises, successfully invested in Chengdu Jiashi Technology, a subsidiary of 29 CLP, and established Haiwei Huaxin Company to enter the field of semiconductor integrated circuit chip manufacturing and build a world-leading compound integrated circuit production line; Sichuan Chuanda Zhisheng Software Co., Ltd. took the shareholding system reform as an opportunity to form a set of mechanism suitable for the industrialization of scientific and technological innovation achievements, featuring the deep integration of Industry-University-Research, and embarked on a new way to transform scientific and technological achievements in colleges and universities into national defense scientific and technological strength; AVIC Chengfei Civil Aircraft not only manufactures the nose for the domestic large aircraft C919, but also actively participates in the development and production of the front and rear boarding gates of Airbus A320 series aircraft …

　　Planning and building characteristic industrial parks to expand the industrial spatial development pattern of integration of defense and civilian technologies.

　　In August last year, a comprehensive strategic cooperation agreement between Chengdu and Beihang University was formally signed, involving seven projects with a total contracted amount of 11.4 billion yuan. According to the agreement, Chengdu, Beihang University, xihua university and other universities and research institutions will give full play to their advantages in location, resources, technology and talents, and strive to build an international innovation port in the west of Beihang University with "one body and two wings".

　　Among them, "integration" means that Beihang cooperates with Chengdu Tianfu New District to focus on building an international innovation port in the west of Beihang, including Beihang Innovation Research Institute, Beihang Innovation Science and Technology Park, Beihang Chengdu Graduate School and Beihang Tianfu International Exchange Center; The "two wings" are to build the Chengdu Aeronautical Power Innovation Research Institute of Beihang University (Blue Power Silicon Valley) and the Chengdu Aeroengine Industrial Base of Beihang University.

　　Industrial park is the carrier support of industrial development. All new projects in cooperation with Beihang University will also be located in integration of defense and civilian technologies Industrial Park planned by Chengdu. At present, Chengdu has started and completed the integration of defense and civilian technologies Innovation Industrial Park in Tianfu New District, integration of defense and civilian technologies Aviation Industrial Base in Xindu District, Pengzhou integration of defense and civilian technologies Aviation Power Industrial Park, and the aviation industrial park construction schemes in Shuangliu District, Hi-tech Zone, Qingyang District and Jintang County have also been completed. The construction of six provincial-level integration of defense and civilian technologies industrial bases undertaken by Chengdu, namely, the complete aircraft industrial base, the nuclear power industrial base, the information security industrial base of China Dianke (Chengdu), the "Yinhe 596" industrial base, the Shuangliu industrial base of the Western Institute and the aerospace high-tech industrial park, are all progressing in an orderly manner.

　　According to the plan, Chengdu will focus on the key areas of integration of defense and civilian technologies’s industrial development, such as aviation, information security, military electronics, nuclear energy and nuclear technology application, mechanical and electrical equipment, new materials and other fields, and plan to build a number of industrial parks with integration of defense and civilian technologies characteristics and industrial bases supported by major projects of leading enterprises, initially forming a spatial development pattern with reasonable layout, multi-park support and coordinated promotion.

　　After years of development, the pattern of mutual support and transformation of military and civilian technologies and capabilities in Chengdu has initially appeared, with the main body of military industry becoming more and more lean, the scientific research capability becoming stronger and stronger, and the level constantly improving; While participate extensively in that scientific research and production of weapons and equipment, civilian enterprise have brought the market mechanism into the field of military industry, showing their ability to compete with the existing military industry, and a scientific research and production system characterized by high technology is bee established.　

IT House May 3 news In response to recent questions from netizens about the reading contract and whether it is completely free, the new team of Reading Group also answered related questions through the official WeChat WeChat official account yesterday.

IT House learned that the contract that attracted widespread attention was launched in September 2019, and the new management team of Reading said that they would communicate with the writers and modify the contract accordingly. In response to the rumor that "all reading is free", the reading team said that this statement is "impossible and unrealistic, please don’t believe it".

Respond to the original:

Dear fellow writers:

Hello everyone! We are very concerned about the reading contract that has been discussed and questioned by everyone since April 28 and whether it is completely free. We also explain it to you here:

1. At present, the contract we are discussing is a contract that was launched in September 2019, not a new contract that was launched on April 28, 2020 as rumored by the outside world.

2. As a new management team on April 27th, 2020, we received many criticisms and opinions on this old contract. We attach great importance to it and have begun to re-examine it. We will have extensive communication with writers, and we will make corresponding amendments to unreasonable terms. Please rest assured.

3. From the beginning of the new team, we firmly believe that we must consolidate and maintain the payment model and explore innovative models. It is impossible and unrealistic to say that "all free reading" is carried out in reading articles rumored by the outside world. Please don’t believe it.

4. Wechat reading is one of the partners of reading channels. About the limited-time free operation of WeChat reading for reading copyright content, which everyone is concerned about, it is the improper operation of the channel itself and the management error of our channel work. We have communicated with WeChat Reading and reached a consensus that the operation activity has been offline and will not have any impact on the author’s income.

5. Writers are the foundation of reading platform. Content ecology is not only for reading, but also for everyone. Now everyone’s opinions and criticisms are not overnight, so it is a very important gain to know everyone’s true feelings. On May 3rd, we will announce the arrangement of a series of talks between the new management and writers, hoping to get your outspoken suggestions on the development of reading and discuss the future together.

After fumbling all the way to today, we have made mistakes and detours. We will face the challenge and hope to create a common future with you.

Cheng Wu, Hou Xiaonan and the New Reading Team

2020-5-2

Disclaimer: This article creates original translated articles for Flint. Individuals are welcome to forward and share them. Reprinting on websites and WeChat official account requires authorization.

I. Literature sources

Taubenberger J　K, Morens D　M. 1918 influenza: the mother of all pandemics[J]. Emerging infectious diseases, 2006, 12(1):15-22.

The literature was published in the American CDC academic journal Emerging Infectious Diseases, January 2006, Volume 12, No.1, with the title "1918 Influenza: Mother of All Pandemic Diseases". （ www.cdc.gov/eid）

II. Introduction to the author

Dr. Taubenberger: Director of Molecular Pathology, United States Army Pathology Institute, Rockville, Maryland. The research direction is the molecular pathophysiology and evolution of influenza virus.

Dr Morens: epidemiologist, working for the American institute of allergy and infectious diseases. His research interests are emerging infectious diseases, virology, tropical medicine and medical history.

foreword

From 1918 to 1919, Spanish influenza caused about 50 million deaths in the world, and it is still an ominous haze of public health. There are still many unanswered questions about the origin, such as unusual epidemiological characteristics and main pathogenic factors. Although we are now trying to overcome the spread of pandemics such as H5N1 or other viruses, the impact of the 1918 influenza on public health is still uncertain. The information of the 1918 influenza is still being updated, such as sequencing the whole genome from the autopsy in the archives, but the virus genome alone cannot answer the key questions. To understand the 1918 flu and its impact on the future, we need detailed experiments and in-depth historical analysis. Tip: The number of words in the full text is 7279, and it takes an average of 15 minutes to finish reading.

background

During the 1918-1919 flu, about one third of the world’s population (about 500 million people) was infected and developed clinical symptoms. The pandemic is more serious than expected. Compared with the mortality rate of other pandemics (< 0.1%), the mortality rate of influenza in 1918 was > 2.5%. It is estimated that the total death toll is about 50 million, and the pessimistic view is as high as 100 million.

The influence of 1918 influenza was not limited to 1918-1919. Since then, almost all influenza A (except avian influenza virus, such as H5N1 and H7N7) in the world are descendants of 1918 influenza virus, including drifting H1N1, recombinant H2N2 and H3N2. The latter is the integration of the key genes of the 1918 influenza by the later avian influenza, thus expressing new proteins. It can be said that the 1918 flu is the mother of all pandemics.In 1918, it was unclear how human influenza was related to avian influenza and swine flu. Although the clinical and epidemiological characteristics are similar to those of influenza pandemics in 1889, 1847 or even earlier, the high mortality rate makes many people wonder whether it should be called influenza. This problem was not solved until 1930, when the influenza virus (now called H1N1) was isolated from pigs, and later it was also isolated from humans. Seroepidemiological studies soon linked the virus with the 1918 flu. Subsequent research shows that the offspring of 1918 virus has always existed in pigs, and may still appear in humans continuously, experiencing the continuous iteration of antigenic drift, and leading to an annual epidemic outbreak until the 1950s. In 1957, a new H _ H2N2 epidemic (namely "Asian influenza") began to appear, and the H1N1 virus, the offspring of the 1918 virus, completely disappeared from the human circulation, although the related pedigree was still attached to pigs. But in 1977, it suddenly "reappeared" from the laboratory freezer and began to be popular among humans.
In 2006, two natural lineages and two recombinant lineages of the offspring of 1918 virus became popular: human H1N1, swine H1N1 (swine flu), recombinant human H3N2 and recombinant swine H3N2, but none of the offspring was close to the pathogenicity of 1918 virus. Swine strains H1N1 and H3N2 rarely infect humans, while the morbidity/mortality of human strains H1N1 and H3N2 are lower than that of 1918 virus.In fact, the fatality rate of natural H1N1 is even lower than that of H3N2 (prevalent since 1968). The H1N1 and H3N2 viruses originated from the 1918 influenza have been prevalent all over the world for 29 years, and there are almost no signs of extinction.

First, try to understand what happened?

By the early 1990s, 75 years of research had failed to answer the most basic question of the 1918 flu: Why was it so deadly? People didn’t isolate any virus from the 1918 influenza epidemic, but their descendants continued to invade humans slightly. A follow-up survey of influenza epidemic in the 1920s showed that in the years after 1918, influenza epidemic became an annual epidemic with low mortality due to gene drift. Did one genetic event greatly reduce the pathogenicity of the 1918 virus, and then another genetic event turned the 1918 virus into a weaker H1N1 virus?

In 1995, a scientific team determined the autopsy materials of influenza in the autumn of 1918 from the archives, and began a long sequencing experiment on small RNA virus fragments, so as to deduce the whole genome of the 1918 virus. At present, the whole genome of one virus and partial gene sequences of four other viruses have been determined. The above experiments and a series of other articles related to 1918 virus have confirmed that 1918 virus is most likely the ancestor of human/pig H1N1, H3N2 and extinct H2N2. No mutation related to the genome of 1918 virus has been found in other highly pathogenic influenza viruses of human or animals, but the continuous study on virulence factors has produced some interesting results. Nevertheless, the 1918 gene sequence data can not answer the origin and epidemiological characteristics of the virus.

Second,When and where did the 1918 flu begin to spread?

Before and after 1918, most influenza pandemics originated in Asia and spread all over the world.During the 12-month period from 1918 to 1919, the 1918 flu spread more or less simultaneously from three regions: Europe, Asia and North America (the first wave is thought to have started in the United States in March 1918). Historical and epidemiological data are not enough to identify the geographical origin of the virus, and the recent 1918 gene system research can not analyze the geographical origin.

Although there was no national influenza disease reporting system in 1918, and the diagnostic criteria for influenza and pneumonia were not clear, since December 1915, there was an epidemic of respiratory diseases in the United States, and the mortality rate of influenza and pneumonia rose sharply in 1915 and 1916, and then decreased slightly in 1917. The first wave of influenza pandemic appeared in the spring of 1918, and the second wave and the third wave of influenza pandemic with higher mortality rate appeared in the autumn of 1918 and the winter of 1919 respectively, as shown in Figure 1.

Is it possible that the weak-adaptability H1N1 virus had spread in 1915, and although there were some serious diseases, it had not caused a pandemic?At that time, data consistent with this was also reported from European military camps, but the opposite conclusion was given: if the virus strain with new hemagglutinin (HA) affected the mortality rate of influenza and pneumonia in the United States, it should have caused a pandemic long ago, and someone should be immunized or at least partially immunized by 1918. It is difficult to determine that the "pioneer" events of the 1918 flu came from 1915, 1916 or even early 1918.

Figure 1 Three waves of epidemics: the weekly mortality rate of influenza and pneumonia in Britain from 1918 to 1919.

Another unique feature of the 1918 influenza pandemic was that people and pigs were infected almost at the same time.The 1918 virus probably expressed a new subtype of antigenicity, which led to the low immunity of most people and pigs. Recently published genetic studies show that hemagglutinin (HA) and neuraminic acid (NA) expressed by the 1918 virus gene were probably derived from the avian influenza virus shortly before the start of the pandemic, and the precursor virus failed to spread widely in humans or pigs for decades. More recent reports of the virus gene sequence and phylogenetic analysis of the virus also support this conclusion. Regression analysis of human/swine flu sequences from 1930 to the present shows that the precursor of 1918 virus first spread in humans from 1915 to 1918. Therefore, the precursor of the 1918 virus may have spread widely among humans before 1918, and so far no research has directly indicated the transition from any bird. In a word, its origin is still confusing.

Are the three waves of epidemics from the same virus?

Historical records since the 16th century show that a new influenza pandemic may occur at any time of the year, which is not necessarily the regular pattern in winter every year, because the behavior pattern most likely from the new influenza has been found among infected people. After that, these pandemic viruses began to drift in the face of the selective pressure of population immunity, and eventually the pattern of annual epidemic recurrence was triggered by drifting virus mutants.

During the 1918-1919 pandemic, the first spring tide began in March 1918 and spread to the United States, Europe and even Asia in different degrees in the next six months. The prevalence rate was very high, but the mortality rate in most areas was not significantly higher than the normal value. The second wave of autumn tide began in September-November, 1918, and it spread rapidly around the world and was fatal. The third wave of epidemic began in early 1919. According to the clinical similarity, some contemporary observers have come to the conclusion at the beginning that the three epidemics should be caused by the same disease: the mild symptoms in the three epidemics are the same, and they have the typical flu characteristics of the 1889 pandemic and earlier pandemics. However, in retrospect, there are few cases in which the first wave of spring tide developed rapidly from simple influenza to fatal pneumonia, and fatal pneumonia is the symbol of the last two waves of epidemic tide; Comparatively speaking, the frequency of complex, serious and fatal cases in the last two waves will be higher.

It is unprecedented for three large-scale epidemics to occur in a year. To some extent, the antigenic drift of the virus is the reason why it keeps recurring every year, and an antigenic virus variant appears about every 2 ~ 3 years. If there is no drift characteristic, once the immunity of human/livestock reaches the critical threshold, the influenza virus may disappear, and under this critical threshold, the further spread of the virus is limited. For decades, people have been speculating about the time and space between influenza pandemics. In addition to human/livestock immune restrictions, the factors of virus transmission include low ambient temperature, nasal cavity temperature (which is beneficial to heat-labile viruses, such as influenza), suitable humidity, indoor congestion and poor ventilation.
However, none of the above factors can explain the three waves of epidemic in 1918-1919, which occurred in spring and summer, summer and autumn and winter respectively. The first two waves occur at the time of unfavorable influenza transmission every year; The second wave broke out in the northern hemisphere/southern hemisphere from September to November; In addition, the period between epidemic tides is so short that it is almost impossible to detect in some areas. It is difficult for epidemiology to explain the sharp decline of cases between the first wave and the second wave, and the sharp rise of cases between the second wave and the third wave. Even assuming that the acquired immunity after infection is short-lived, there are too few susceptible people to maintain the spread at one point. How can a new explosive pandemic be triggered in a few weeks? Will the virus mutate completely at the same time in almost a short time around the world?
However, according to scientific experience, it takes several years for influenza virus to spread globally, not just a few weeks. Moreover, even the mutant that has drifted will take several months to spread around the world. Other influenza pandemics have not been reported continuously within one year. For example, the 1889 flu began in the late spring of 1889, and it took several months to spread around the world, reaching its peak in northern Europe and the United States in late 1889 or early 1890; The second recurrence occurred in late spring of 1891 (one year late) and the third in early 1892. Like the 1918 flu, more people died when the second epidemic wave recurred, but the spread time of the three epidemics in 1889-1892 was more than three years, while the spread time of the successive epidemics in 1918-1919 was compressed to August-September.The ability of the 1918 influenza to rapidly develop a continuous pandemic is still unknown. Because the autopsy materials we identified only came from the second wave of 1918 influenza, it is inconclusive whether the first wave or the third wave also came from the same virus or mutant. The experimental data show that the second wave of infected people may have obtained immune protection during the third wave of epidemic, but the data is not enough to support whether the second/third wave of infected people obtained immune protection from the first wave of epidemic, nor can they answer the question whether the three waves of epidemic came from the same virus or mutant. Only by getting the RNA positive samples of infected people before 1918 and the three waves of epidemic in 1918 can we answer this question.

4. Did the 1918 virus host come from animals?

The data of 1918 virus gene sequence shows that the whole genome may be new to human beings in 1918 or not long ago. It was not produced by recombining one or more new genes from previous viruses (unlike the 1957 or 1968 epidemic). On the contrary, the 1918 virus seems to be derived from an unknown source of avian influenza virus (eight gene fragments are different from the current avian influenza genes). The gene sequence of influenza virus collected from wild bird specimens around 1918 is not much different from that of today’s avian influenza virus, which indicates that even for a long time, the antigenic changes of avian influenza virus may hardly occur in natural reservoir.

For example, the sequence of 1918 nucleoprotein (NP) gene is similar to the virus in wild birds in amino acid level, but it is very different in nucleotide level, which indicates that the source of 1918 nucleoprotein is far from the virus nucleoprotein in wild birds at present. One way to check the evolutionary distance of genes is to compare the ratio of synonymous nucleotides to non-synonymous nucleotides. Synonymous substitution means that the nucleotide change in the sequence will not lead to amino acid substitution; Non-synonymous substitution means that nucleotide changes will lead to amino acid substitution. Generally speaking, viruses under immune drift pressure or adapting to new hosts show a larger proportion of non-synonymous mutations, while viruses under minimal pressure mainly accumulate synonymous substitutions, which are considered to reflect the evolutionary distance.

Because the 1918 influenza virus gene fragments known from wild birds have more synonymous substitutions than expected, they are unlikely to come directly from viruses similar to avian influenza virus. One of them is particularly obvious. When people check the 4-fold degenerate codons (a subset of synonymous substitutions), they find that the third codon can replace any of the 4 nucleotides without changing the amino acids. At the same time, although the 1918 virus has been adapted to the intermediate host of human/pig for many years, the amino acid level of its sequence is still very small compared with that of wild birds. One possible explanation is that these abnormal gene fragments were obtained from an unidentified influenza virus database.All these findings lead to a question: Where did the 1918 virus come from?

Contrary to the 1918 virus, the gene fragments of the recombinant viruses of 1957 and 1968 influenza were from Eurasian avian influenza virus. Both human viruses are produced by the same mechanism: it is the recombination of Eurasian avian influenza virus and the previously popular human H1N1 virus. To prove the origin of the 1918 virus, samples more similar to the 1918 virus sequence are needed: samples before the 1918 virus epidemic and wild epidemic samples.

5. What is the biological basis of the pathogenicity of the 1918 virus?

Single gene sequence analysis can not provide the cause of pathogenicity of 1918 virus. Through a series of in vitro/animal model experiments, the researchers tried to model the virulence of the virus constructed by reverse gene.

Influenza virus infection requires HA protein to bind to sialic acid receptors on the surface of host cells.However, the configuration of HA receptor binding site of influenza virus suitable for avian infection is different from that of influenza virus suitable for human infection. Avian influenza virus preferentially binds sialic acid receptor with α(2-3) linked sugar; Human influenza virus is considered to preferentially use α(2-6) linked sugar. This avian receptor configuration can be transformed into infection only by changing one amino acid, while the HA protein of five sequenced 1918 viruses has this change, which is probably a key step to adapt to human hosts. There is also a second possibility that three of the five sequenced 1918 viruses have another change in their HA sequence that enhances the binding of human receptors.

This means that at least two H1N1 receptor-binding mutants spread in 1918: one shows high affinity with human receptor and the other shows mixed high affinity with avian/human receptor. But there is no geographical or chronological indication that one of the variants is the precursor of the other.Did these viruses have the same transmission ability in 1918, did they have the same replication pattern in the respiratory tree, and did they spread the same virus in the first and third epidemics? Are unknown.

In a series of in vivo experiments, recombinant genes containing gene fragments 1 and 5 of 1918 virus have been produced. These recombinants with 1918 HA/NA showed high pathogenicity in mice. In addition, the microarray analysis of gene expression in lung tissue of these mice showed that cell apoptosis, tissue damage and oxidation were all related to the expression of such genes. These findings are unexpected, because the virus with 1918 gene has not adapted to mice; The control group (mice infected with other modern human viruses) showed little pathogenicity and virus replication. Mice infected with 1918 HA/NA recombinant showed necrosis of bronchial and alveolar epithelium and obvious inflammatory infiltration in their lungs, which indicated that 1918 HA (and possibly NA) contained pathogenic factors for mice. It is not clear whether the pathogenicity of mice can effectively simulate the pathogenicity of humans, and the potential role of other 1918 single or combined proteins is not clear. The researchers plan to further draw the genetic basis of the toxicity of the 1918 virus on other animal models. These experiments may help to determine the pathogenic components of the 1918 virus, but it is impossible to determine whether it is the main factor in the death pattern of the 1918 influenza.

Why did the 1918 virus kill so many healthy young people?

From the historical experience, the flu death curve by age has been U-shaped for at least 150 years (Figure 2), which shows the peak of death in childhood and old age, and the death rate in the middle age group is lower. On the contrary, the 1918 flu showed a unique pattern, a "W-shaped" curve that had never been recorded, and there was another death peak among people aged about 20-40.During 1918-1919, the mortality rate of people aged 15-34 suffering from influenza and pneumonia was more than 20 times higher than in previous years. Nearly half of the 1918 flu deaths came from people aged 20-40, which was unique to the 1918 flu.

Another unique point is that the mortality rate of people under 65 years old is much higher than that of people over 65 years old, and it accounts for 99% of the flu deaths in 1918. In comparison, the mortality rate of people under 65 years old accounted for 36% of the 1957 H2N2 influenza and 48% of the 1968 H3N2 influenza.

　

Figure 2 Mortality rate of influenza and pneumonia per 100,000 population of all ages in the United States from 1911 to 1918.

Further dividing the influenza mortality curve by age, we can clearly see that the prevalence rate of people under 35 years old is very high (Figure 3-a), but there is still a W-shaped death peak among young people, which is obviously different from the U-shaped curve of influenza in 1928-1929 (Figure 3-c). The same 1918 flu, the prevalence rate of 5 ~ 14 years old is also high, but the mortality rate is much lower (Figure 3-a/b). In order to explain this model, we must jump out of the host and environmental factors and look further, which may include immunopathological factors (such as increased antibody-dependent infection associated with virus exposure) and other risk factors (such as concurrent infection, drugs used and environmental factors). See figure 3.

Fig. 3 Community survey results of American public health institutions in 8 states in 1918.
Note: Figures A, B and C show the prevalence rate, mortality rate and mortality rate of influenza and pneumonia in different age groups respectively.

There is a theory that can partially explain these findings. The 1918 virus is highly toxic, but for patients born before 1889, that is, people who are over 35 years old and have been exposed to the virus at that time, they have obtained partial immune protection against the 1918 virus. But there is a paradox in this theory: this nameless virus precursor must have appeared before 1889, and then reappeared 30 years later, but no trace has been found today.

Epidemiological data collected from 1900 to 1918 provided good evidence for the emergence of a new antigenic virus in 1918. It shows that from 1900 to 1917, the age group of 5 to 15 years old accounted for 11% of the total number of influenza cases, while the age group over 65 years old accounted for 6% of the total number of influenza cases. However, in 1918, the age group of 5-15 years jumped to 25% of the total number of influenza cases, while the age group over 65 years accounted for only 0.6% of the total number of influenza cases. This is consistent with the results of protective immunity obtained by previous exposure to the same or related viruses, and the mortality data is also consistent. In 1918, the number of deaths from influenza and pneumonia in the age group over 75 was less than that in 1911-1917 before the pandemic. At the other end of the age group (Figure 2), the infant mortality rate in 1918 was similar to other pandemic patterns.

Will the 1918 flu reappear? If so, what should I do?

As far as the course of disease and pathology are concerned, the 1918 influenza pandemic is different from the historical pandemic only in degree, not in species.Despite the large number of deaths worldwide, most infected people (> 95% from industrialized countries) are mild, which is not much different from today’s influenza. In addition, the experimental results of recombinant influenza virus containing 1918 virus gene show that 1918 virus and 1918-like virus are as sensitive to the anti-influenza drugs rimantadine and oseltamivir (approved by FDA) as other typical influenza viruses.

However, the 1918 flu has some characteristics of its own: in particular, the mortality rate is 5 ~ 20 times higher than expected. From the clinical and pathological point of view, these high mortality rates seem to be caused by many factors, including a higher proportion of severe respiratory infections (organ failure not caused by influenza); In addition, death cases are concentrated in an unusually young age group; Finally, in 1918, three influenza epidemics appeared one after another at an unusually rapid speed. Each feature may reflect the genetic characteristics of the 1918 virus, but to understand them, it is necessary to check the host and environment.

Until we can’t determine which factors lead to the influenza pandemic and high mortality rate, these can only be guesses. We can only come to the conclusion that since it has happened before, similar conditions may lead to the same serious pandemic.

Like the virus in 1918, H5N1 is an avian virus, although it is a distant relative. The reasons for the evolutionary path of the 1918 pandemic are completely unknown, but it seems to be different from the current H5N1 situation in many aspects. No matter the 1918 pandemic or any other infectious disease pandemic, there is no historical data showing the existence of a pandemic "precursor virus", which led to the outbreak of highly pathogenic infection in poultry; There is no historical data to prove that highly pathogenic avian influenza (HPAI) virus, including other virus subtypes such as H5N1, can cause human epidemics, let alone pandemics.

Effective human-to-human transmission is the main prerequisite for the emergence of pandemic.Although the adaptation of influenza viruses to human cells (such as receptor binding) has begun to be understood at the molecular level, how various influenza viruses adapt to humans is not completely clear at present. In 1918, the virus acquired the characteristics of adapting to human beings, but at present, we have no way to know whether the H5N1 virus will acquire the ability of human-to-human transmission in a parallel process. Although the data about the 1918 virus has increased geometrically in the past ten years, we don’t know much more about the 2006 influenza pandemic than about the risk of the 1976 H1N1 "swine flu" epidemic.

Even with modern antiviral drugs, vaccines, and knowledge of infectious disease prevention, if there is another virus like 1918, it may still kill more than 100 million people all over the world. An outbreak of a pandemic virus with high pathogenicity potential, such as the recent H5N1 virus, may cause more deaths.

No matter the virus, host or environmental factors, the virus of the first wave in 1918 had nothing to do with the abnormal pathogenicity of the second and third waves. And more, identify the virulence models of the first wave of influenza RNA positive cases from different gene sequences; Identification of human influenza RNA samples before 1918 is helpful to understand the time when the 1918 virus appeared; The observation and sequencing of a large number of animal influenza viruses will help us to understand the genetic basis of adapting to the host and the natural reservoir range of influenza. Generally speaking, it is necessary to have a comprehensive understanding of the 1918 influenza from the aspects of history, epidemiology and biology in order to answer many unsolved mysteries.

Acknowledgement:This article was reviewed by Dr. Zhu Xun, a famous immunologist and an expert of the former National New Drug Advisory Committee. I sincerely thank Mr. Zhu for his careful guidance!

Translation: Shi Junxin　

Editor: Ginger